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one-color microarray hybridization protocol, v6.5  (Agilent technologies)


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    Agilent technologies one-color microarray hybridization protocol, v6.5
    One Color Microarray Hybridization Protocol, V6.5, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/microarray+hybridization+protocols/pm24704562-89-23-24
    Average 90 stars, based on 1 article reviews
    one-color microarray hybridization protocol, v6.5 - by Bioz Stars, 2026-11
    90/100 stars

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    Related Articles

    Hybridization:

    Article Title: MiR-650 represses high-risk non-metastatic colorectal cancer progression via inhibition of AKT2/GSK3β/E-cadherin pathway
    Article Snippet: .. The labelling and hybridization were performed according to protocols in the Agilent microRNA microarray system. .. The labelling and hybridization were performed according to protocols in the Agilent microRNA microarray system.

    Article Title: Targeting abnormal DNA repair in therapy-resistant breast and pancreatic cancers
    Article Snippet: .. After hybridization to microarrays for 40 hours at 65° C. in a rotating oven, the microarrays were washed and dried according to the manufacturer's protocols, and then imaged with dan Agilent G2565BA microarray scanner. .. Data were extracted with Feature Extraction Software (v9.5.3.1; Agilent Technologies) and analyzed by Agilent's Genomic Workbench v 5.0.

    Article Title: Targeting abnormal DNA repair in therapy-resistant breast and pancreatic cancers
    Article Snippet: .. After hybridization to microarrays for 40 hours at 65° C. in a rotating oven, the microarrays were washed and dried according to the manufacturer's protocols, and then imaged with dan Agilent G2565BA microarray scanner. .. Data were extracted with Feature Extraction Software (v9.5.3.1; Agilent Technologies) and analyzed by Agilent's Genomic Workbench v 5.0.

    Article Title: Platelet microRNA-15b protects against high platelet reactivity in patients undergoing percutaneous coronary intervention through Bcl-2-mediated platelet apoptosis
    Article Snippet: MicroRNA microarray and data analysis To determine the diverse miRNA expression in platelets between LPR and HPR patients, we performed miRNA expression profiles of LDP samples from four LPR and four HPR patients using Agilent human miRNA microarray (version 19.0) system (Agilent technologies, Santa Clara, CA, USA). .. The miRNA molecular labeling, array hybridization and data scanning were performed at Biochip Company (Shanghai, China), according to the protocols of the Agilent miRNA microarray system. .. Raw data were normalized by Quantile algorithm, Gene Spring Software 11.0. miRNA microarray data has been deposited in the National Center for Biotechnology Information Gene Expression Omnibus ( http://www.ncbi.nlm.nih.gov/geo/ ), with the accession number GSE59488 .

    Article Title: Meta-microRNAs as potential noninvasive markers for early diagnosis of Alzheimer’s disease
    Article Snippet: .. The labeling and hybridization were performed according to the protocols in the Agilent microRNA microarray system. ..

    Article Title: Systematic integration of molecular and clinical approaches in HCV-induced hepatocellular carcinoma
    Article Snippet: .. Hybridization and labeling were performed according to the protocols in the Agilent miRNA microarray system. .. Microarray images were analyzed with Feature Extraction Software (Agilent).

    Microarray:

    Article Title: MiR-650 represses high-risk non-metastatic colorectal cancer progression via inhibition of AKT2/GSK3β/E-cadherin pathway
    Article Snippet: .. The labelling and hybridization were performed according to protocols in the Agilent microRNA microarray system. .. The labelling and hybridization were performed according to protocols in the Agilent microRNA microarray system.

    Article Title: Targeting abnormal DNA repair in therapy-resistant breast and pancreatic cancers
    Article Snippet: .. After hybridization to microarrays for 40 hours at 65° C. in a rotating oven, the microarrays were washed and dried according to the manufacturer's protocols, and then imaged with dan Agilent G2565BA microarray scanner. .. Data were extracted with Feature Extraction Software (v9.5.3.1; Agilent Technologies) and analyzed by Agilent's Genomic Workbench v 5.0.

    Article Title: Targeting abnormal DNA repair in therapy-resistant breast and pancreatic cancers
    Article Snippet: .. After hybridization to microarrays for 40 hours at 65° C. in a rotating oven, the microarrays were washed and dried according to the manufacturer's protocols, and then imaged with dan Agilent G2565BA microarray scanner. .. Data were extracted with Feature Extraction Software (v9.5.3.1; Agilent Technologies) and analyzed by Agilent's Genomic Workbench v 5.0.

    Article Title: Platelet microRNA-15b protects against high platelet reactivity in patients undergoing percutaneous coronary intervention through Bcl-2-mediated platelet apoptosis
    Article Snippet: MicroRNA microarray and data analysis To determine the diverse miRNA expression in platelets between LPR and HPR patients, we performed miRNA expression profiles of LDP samples from four LPR and four HPR patients using Agilent human miRNA microarray (version 19.0) system (Agilent technologies, Santa Clara, CA, USA). .. The miRNA molecular labeling, array hybridization and data scanning were performed at Biochip Company (Shanghai, China), according to the protocols of the Agilent miRNA microarray system. .. Raw data were normalized by Quantile algorithm, Gene Spring Software 11.0. miRNA microarray data has been deposited in the National Center for Biotechnology Information Gene Expression Omnibus ( http://www.ncbi.nlm.nih.gov/geo/ ), with the accession number GSE59488 .

    Article Title: Meta-microRNAs as potential noninvasive markers for early diagnosis of Alzheimer’s disease
    Article Snippet: .. The labeling and hybridization were performed according to the protocols in the Agilent microRNA microarray system. ..

    Article Title: Systematic integration of molecular and clinical approaches in HCV-induced hepatocellular carcinoma
    Article Snippet: .. Hybridization and labeling were performed according to the protocols in the Agilent miRNA microarray system. .. Microarray images were analyzed with Feature Extraction Software (Agilent).

    In Situ Hybridization:

    Article Title: The effects of dietary curcumin and rutin on colonic inflammation and gene expression in multidrug resistance gene-deficient (mdr1a−/−) mice, a model of inflammatory bowel diseases
    Article Snippet: .. Labelled cRNA were hybridised using an In Situ Hybridization Plus Kit (Agilent Technologies), following the manufacturer’s protocols, using 750 ng of experimental and reference cRNA onto 44K mouse oligonucleotide arrays (Agilent Technologies). .. After hybridisation, slides were washed in solutions I, II and III (Agilent Technologies) and air-dried.

    other:

    Article Title: Everolimus-induced epithelial to mesenchymal transition in immortalized human renal proximal tubular epithelial cells: key role of heparanase
    Article Snippet: After hybridization the slides were washed according to Agilent protocols and finally scanned using the High-Resolution Microarray C Scanner (Agilent Technologies).

    Labeling:

    Article Title: Platelet microRNA-15b protects against high platelet reactivity in patients undergoing percutaneous coronary intervention through Bcl-2-mediated platelet apoptosis
    Article Snippet: MicroRNA microarray and data analysis To determine the diverse miRNA expression in platelets between LPR and HPR patients, we performed miRNA expression profiles of LDP samples from four LPR and four HPR patients using Agilent human miRNA microarray (version 19.0) system (Agilent technologies, Santa Clara, CA, USA). .. The miRNA molecular labeling, array hybridization and data scanning were performed at Biochip Company (Shanghai, China), according to the protocols of the Agilent miRNA microarray system. .. Raw data were normalized by Quantile algorithm, Gene Spring Software 11.0. miRNA microarray data has been deposited in the National Center for Biotechnology Information Gene Expression Omnibus ( http://www.ncbi.nlm.nih.gov/geo/ ), with the accession number GSE59488 .

    Article Title: Meta-microRNAs as potential noninvasive markers for early diagnosis of Alzheimer’s disease
    Article Snippet: .. The labeling and hybridization were performed according to the protocols in the Agilent microRNA microarray system. ..

    Article Title: Systematic integration of molecular and clinical approaches in HCV-induced hepatocellular carcinoma
    Article Snippet: .. Hybridization and labeling were performed according to the protocols in the Agilent miRNA microarray system. .. Microarray images were analyzed with Feature Extraction Software (Agilent).



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    Image Search Results


    (A) Control and IFIT1-depleted THP1 cells were stimulated with 100 ng/mL LPS for 1, 2, 4, 6, and 24 hr, and transcriptional responses were measured by microarray. The upper heatmap shows genes with substantial expression increases in WT cells compared to their expression level in IFIT1-depleted cells. The lower heatmap shows the sum of the expression difference between WT and IFIT1-depleted cells across the time course . (B and C) The network connections of genes that showed (B) enhanced expression (group 1; ) or (C) reduced expression (group 2; ) in IFIT1-depleted cells, as determined by IPA. (D) Transfection of siRNA into IFIT1 shRNA-expressing cells sustains a stable suppression of IFIT1 induction in response to 100 ng/mL LPS. (E and F) Control and IFIT1-depleted THP1 cells (E) or human primary macrophages (F) were stimulated with 100 ng/mL LPS, and secreted IFN-β protein was measured by ELISA. Data are representative of two (A) or three (D–F) independent experiments. Data in (D)–(F) are expressed as mean SD; *p < 0.05, **p < 0.01 (two-way ANOVA followed by Sidak’s multiple comparison test). See also and , , and .

    Journal: Cell reports

    Article Title: IFIT1 Exerts Opposing Regulatory Effects on the Inflammatory and Interferon Gene Programs in LPS-Activated Human Macrophages

    doi: 10.1016/j.celrep.2018.09.002

    Figure Lengend Snippet: (A) Control and IFIT1-depleted THP1 cells were stimulated with 100 ng/mL LPS for 1, 2, 4, 6, and 24 hr, and transcriptional responses were measured by microarray. The upper heatmap shows genes with substantial expression increases in WT cells compared to their expression level in IFIT1-depleted cells. The lower heatmap shows the sum of the expression difference between WT and IFIT1-depleted cells across the time course . (B and C) The network connections of genes that showed (B) enhanced expression (group 1; ) or (C) reduced expression (group 2; ) in IFIT1-depleted cells, as determined by IPA. (D) Transfection of siRNA into IFIT1 shRNA-expressing cells sustains a stable suppression of IFIT1 induction in response to 100 ng/mL LPS. (E and F) Control and IFIT1-depleted THP1 cells (E) or human primary macrophages (F) were stimulated with 100 ng/mL LPS, and secreted IFN-β protein was measured by ELISA. Data are representative of two (A) or three (D–F) independent experiments. Data in (D)–(F) are expressed as mean SD; *p < 0.05, **p < 0.01 (two-way ANOVA followed by Sidak’s multiple comparison test). See also and , , and .

    Article Snippet: Each condition was represented by two biological replicates. cRNA amplification and labeling were performed using the Illumina TotalPrep RNA Amplification Kit (Ambion), microarray hybridization and scanning protocols followed standard Illumina protocols.

    Techniques: Microarray, Expressing, Transfection, shRNA, Enzyme-linked Immunosorbent Assay

    KEY RESOURCES TABLE

    Journal: Cell reports

    Article Title: IFIT1 Exerts Opposing Regulatory Effects on the Inflammatory and Interferon Gene Programs in LPS-Activated Human Macrophages

    doi: 10.1016/j.celrep.2018.09.002

    Figure Lengend Snippet: KEY RESOURCES TABLE

    Article Snippet: Each condition was represented by two biological replicates. cRNA amplification and labeling were performed using the Illumina TotalPrep RNA Amplification Kit (Ambion), microarray hybridization and scanning protocols followed standard Illumina protocols.

    Techniques: Recombinant, Microarray, shRNA, Software